Does high-mobility-group non-histone protein HMG 1 interact specifically with histone H1 subfractions?

Does high-mobility-group non-histone protein HMG 1 interact specifically with histone H1 subfractions?

The interaction of the non-histone chromosomal protein HMG (high-mobility group) 1 with histone H1 subfractions was investigated by equilibrium sedimentation and n.m.r. sectroscopy. In contrast with a previous report [Smerdon & Isenberg (1976) Biochemistry 15, 4242--4247], it was found, by using equilibrium-sedimentation analysis, that protein HMG 1 binds to all three histone H1 subfractions CT...

The isolation and identification of ubiquitin from the high mobility group (HMG) non-histone protein fraction.

Chromatin contains a group of non-histone chromosomal proteins called the high mobility group (HMG) proteins [l] . Four of the HMG proteins from calf thymus, HMG 1,2,14 and 17, and an HMG protein from trout testis, HMG-T, have been isolated in a pure form [2-51. The amino acid sequence of calf thymus HMG 17 has recently been determined [6] , and we have also shown that HMG proteins HMG 1,2, 14 ...

Pairing of DNA fragments containing (GGA:TCC)n repeats and promotion by high mobility group protein 1 and histone H1.

Tandemly repeated DNA sequences of (GGA:TCC)n are found in tracts up to 50 base pairs long, dispersed at thousands of sites throughout the genomes of eukaryotes. Here we demonstrate the formation of complexes paired between two DNAs containing such repeats in vitro and show enhancement of the pairing by glutathione S-transferase fusion proteins of high mobility group protein 1 and histone H1. T...

Effect of high mobility group nonhistone proteins HMG-20 (ubiquitin) and HMG-17 on histone deacetylase activity assayed in vitro.

We have used a method previously described by Reeves and Candido (1) to partially release histone deacetylase from cell nuclei together with putative regulators of the enzyme. Histone deacetylase released from testis cell nuclei and its putative regulators were separated by gel filtration in Sepharose 6B. A peak of low molecular weight contains a heat-stable factor that stimulate histone deacet...

HMGIY (high mobility group protein (non histone chromosomal) isoform I and Y)